The following techniques are being developed in the laboratory. We have acquired the equipment and resources and are currently running the first set of pilot experiments.

Ex vivo calcium imaging

sCMOS imaging of cells transduced with GCaMP6 

Prime sCMOS camera from qImaging

Prime sCMOS camera from qImaging

Fig 5.png
 

In vivo fiber photometry

Tucker-Davis turn-key fiber photometry set-up.

Tucker-Davis turn-key fiber photometry set-up.

Chat-IRES-Cre+/-  injected with AAV5-GCaMP6f virus and implanted with a photometry fiber in the NAc.

Chat-IRES-Cre+/- injected with AAV5-GCaMP6f virus and implanted with a photometry fiber in the NAc.